DENND1B Protein (His tag)
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- Target See all DENND1B Proteins
- DENND1B (DENN/MADD Domain Containing 1B (DENND1B))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This DENND1B protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human DENND1B (full length, N-term HIS tag, transcript variant 2) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product DENND1B Protein
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DENN/MADD Domain Containing 1B (DENND1B) (AA 1-396) protein (GST tag)
DENND1B Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
DENN/MADD Domain Containing 1B (DENND1B) (AA 1-775) protein (Strep Tag)Crystallography grade DENND1B Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
DENN/MADD Domain Containing 1B (DENND1B) (AA 1-766) protein (Strep Tag)Crystallography grade DENND1B Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- DENND1B (DENN/MADD Domain Containing 1B (DENND1B))
- Alternative Name
- Dennd1b (DENND1B Products)
- Synonyms
- C1ORF18 Protein, C1orf218 Protein, FAM31B Protein, 4632404N19Rik Protein, 4930467M19Rik Protein, 6820401H01Rik Protein, F730008N07Rik Protein, Fam31b Protein, si:ch211-195i21.1 Protein, DENN domain containing 1B Protein, DENN/MADD domain containing 1B Protein, DENND1B Protein, dennd1b Protein, Dennd1b Protein
- Background
- Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2 see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010].
- Molecular Weight
- 44.8 kDa
- NCBI Accession
- NP_659414
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