DPH5 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
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- Target See all DPH5 Proteins
- DPH5 (DPH5 Homolog (DPH5))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 2
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DPH5 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Diphthine synthase (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product DPH5 Protein
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DPH5 Homolog (DPH5) (AA 1-285) protein (GST tag)
DPH5 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
DPH5 Homolog (DPH5) (AA 1-285) protein (Strep Tag)Crystallography grade DPH5 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
DPH5 Homolog (DPH5) (AA 1-281) protein (Strep Tag)Crystallography grade DPH5 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- DPH5 (DPH5 Homolog (DPH5))
- Alternative Name
- Diphthine Synthase (DPH5 Products)
- Synonyms
- CGI-30 Protein, HSPC143 Protein, NPD015 Protein, 2410012M04Rik Protein, AU045680 Protein, C80186 Protein, diphthamide biosynthesis 5 Protein, DPH5 Protein, Dph5 Protein
- Background
- This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene.
- Molecular Weight
- 31.5 kDa
- NCBI Accession
- NP_057042
- Pathways
- SARS-CoV-2 Protein Interactome
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