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DNMT3A Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)

DNMT3A Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
Catalog No. ABIN2719617
  • Target See all DNMT3A Proteins
    DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    Transcript Variant 3
    Origin
    • 6
    • 1
    Human
    Source
    • 3
    • 2
    • 2
    HEK-293 Cells
    Purification tag / Conjugate
    This DNMT3A protein is labelled with Myc-DYKDDDDK Tag.
    Application
    Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
    Specificity
    Optimal preservation of protein structure, post-translational modifications and functions.
    Characteristics
    • Recombinant human DNMT3A (transcript variant 3) protein expressed in HEK293 cells.
    • Produced with end-sequenced ORF clone
    • Tested for bioactivity.
    Purity
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
    Biological Activity Comment
    DNMT3A activity verified in a biochemical assay:,DNMT3A activity verified in a biochemical assay:,DNMT3A activity verified in a biochemical assay:
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays
    Comment

    The tag is located at the C-terminal.

    Restrictions
    For Research Use only
  • Concentration
    > 50 μg/mL
    Buffer
    25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Cree, Fredericks, Miller, Pearce, Filichev, Fee, Kennedy: "DNA G-quadruplexes show strong interaction with DNA methyltransferases in vitro." in: FEBS letters, Vol. 590, Issue 17, pp. 2870-83, (2016) (PubMed).

  • Target
    DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))
    Alternative Name
    Dnmt3a (DNMT3A Products)
    Synonyms
    DNMT3A Protein, DNMT3A2 Protein, M.HsaIIIA Protein, MmuIIIA Protein, dnmt3a1 Protein, dnmt6 Protein, DNA methyltransferase 3 alpha Protein, DNA methyltransferase 3A Protein, DNA (cytosine-5-)-methyltransferase 3 alpha b Protein, DNMT3A Protein, Dnmt3a Protein, dnmt3a Protein, dnmt3ab Protein
    Background
    CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
    Molecular Weight
    101.7 kDa
    NCBI Accession
    NP_072046
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