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E2F1 Protein (Myc-DYKDDDDK Tag)

E2F1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Catalog No. ABIN2719894
  • Target See all E2F1 Proteins
    E2F1 (E2F Transcription Factor 1 (E2F1))
    Protein Type
    Recombinant
    Origin
    • 4
    • 2
    • 1
    • 1
    • 1
    Human
    Source
    • 2
    • 2
    • 2
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This E2F1 protein is labelled with Myc-DYKDDDDK Tag.
    Application
    Antibody Production (AbP), Standard (STD)
    Characteristics
    • Recombinant human E2F1 protein expressed in HEK293 cells.
    • Produced with end-sequenced ORF clone
    Purity
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
    Top Product
    Discover our top product E2F1 Protein
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Comment

    The tag is located at the C-terminal.

    Restrictions
    For Research Use only
  • Concentration
    50 μg/mL
    Buffer
    25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Tarangelo, Lo, Teng, Kim, Le, Watson, Furth, Raman, Ehmer, Viatour: "Recruitment of Pontin/Reptin by E2f1 amplifies E2f transcriptional response during cancer progression." in: Nature communications, Vol. 6, pp. 10028, (2015) (PubMed).

  • Target
    E2F1 (E2F Transcription Factor 1 (E2F1))
    Alternative Name
    e2f1 (E2F1 Products)
    Synonyms
    CG6376 Protein, DRTF1/E2F Protein, DmE2F-1 Protein, Dmel\\CG6376 Protein, Dp Protein, E(Sev-CycE)3A Protein, E(var)3-93E Protein, E(var)3-95E Protein, E(var)93E Protein, E2F Protein, E2F-1 Protein, E2F1 Protein, E2f1 Protein, Evar(3)164 Protein, dE2F Protein, dE2F1 Protein, dE2f Protein, dE2f1 Protein, de2f1 Protein, def21 Protein, drosE2F1 Protein, e2f Protein, e2f1 Protein, l(3)07172 Protein, l(3)j3B1 Protein, l(3)j3C2 Protein, l(3)rM729 Protein, RBAP1 Protein, RBBP3 Protein, RBP3 Protein, mKIAA4009 Protein, xE2F Protein, ATE2FB Protein, E2F TRANSCRIPTION FACTOR-1 E2F1 Protein, E2F transcription factor 1 Protein, E2FB Protein, E2FB TRANSCRIPTION FACTOR Protein, E2F transcription factor 1 Protein, E2F transcription factor 1 L homeolog Protein, E2f1 Protein, E2F1 Protein, e2f1.L Protein
    Background
    The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
    Molecular Weight
    46.7 kDa
    NCBI Accession
    NP_005216
    Pathways
    p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
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