EARS2 Protein (His tag)
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- Target See all EARS2 Proteins
- EARS2 (Glutamyl-tRNA Synthetase 2 Mitochondrial (EARS2))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This EARS2 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human EARS2 (full length, N-term HIS tag, transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product EARS2 Protein
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Glutamyl-tRNA Synthetase 2 Mitochondrial (EARS2) (AA 1-523) protein (GST tag)
EARS2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Glutamyl-tRNA Synthetase 2 Mitochondrial (EARS2) (AA 42-523) protein (His tag)Crystallography grade EARS2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Glutamyl-tRNA Synthetase 2 Mitochondrial (EARS2) (AA 42-523) protein (His tag)Crystallography grade EARS2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- EARS2 (Glutamyl-tRNA Synthetase 2 Mitochondrial (EARS2))
- Alternative Name
- Ears2 (EARS2 Products)
- Synonyms
- mse1 Protein, 3230401I01Rik Protein, AL024049 Protein, mKIAA1970 Protein, COXPD12 Protein, MSE1 Protein, RGD1307904 Protein, ears2 Protein, glutamyl-tRNA synthetase 2, mitochondrial Protein, zgc:153247 Protein, EARS2 Protein, ears2 Protein, Ears2 Protein, zgc:153247 Protein
- Background
- This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of glutamate to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 12 (COXPD12). Alternative splicing results in multiple transcript variants.
- Molecular Weight
- 58.5 kDa
- NCBI Accession
- NP_001077083
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