EBI3 Protein (His tag)

Catalog No. ABIN2719909

Product Details

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Purification tag / Conjugate: This EBI3 protein is labelled with His tag.

Application: Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human EBI3 / IL27B protein expressed in CHO cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Endotoxin Level: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 60 - 90 ng/ml, corresponding to a specific activity of 1.1 - 1.7 x 10^4 units/mg, determined by mouse splenocytes IL-2 inhibition induced by hIL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 20 mM NaHPO4, pH 6, 0.60 M NaCl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Wang, Wei, Xiao, Liu, Zhang, Han, Chen, Hou, Ma, Shen, Li, Egwuagu, Wang: "A novel IL-23p19/Ebi3 (IL-39) cytokine mediates inflammation in Lupus-like mice." in: European journal of immunology, Vol. 46, Issue 6, pp. 1343-50, (2016) (PubMed).

Target Details

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Alternative Name: Ebi3,il27b (IL-27b Products)

Synonyms: IL-27B Protein, IL27B Protein, EBI3 Protein, il-27rb Protein, si:dkey-97a13.4 Protein, EBI-3 Protein, IL-27 Protein, Epstein-Barr virus induced 3 Protein, Epstein-Barr virus induced gene 3 Protein, EBI3 Protein, ebi3 Protein, Ebi3 Protein

Target Type: Viral Protein

Background: This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells.

Molecular Weight: 50 kDa

NCBI Accession: NP_005746