FBLN3 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719970

Product Details

Target: FBLN3 (Fibulin 3 (FBLN3))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FBLN3 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: : 200 ng of recombinant purified EFEMP1-myc were added to cultured glioma cells (CNS-1 rat glioma cell line) for 6h, and cells were further processed for quantitative RT-PCR. Results showed increased expression of metalloproteases MMP2 and MMP9, in agreement with published results from Hu. et. al. (Mol. Cancer Res. (2009) 7:1756-1770) Data kindly provided by Dr. Mariano S. Viapiano, Ohio State University.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Nandhu, Hu, Cole, Erdreich-Epstein, Rodriguez-Gil, Viapiano: "Novel paracrine modulation of Notch-DLL4 signaling by fibulin-3 promotes angiogenesis in high-grade gliomas." in: Cancer research, Vol. 74, Issue 19, pp. 5435-48, (2014) (PubMed).

Target Details

Target: FBLN3 (Fibulin 3 (FBLN3))

Alternative Name: Egf-Containing Fibulin-Like Extracellular Matrix Protein 1 (Efemp1) (FBLN3 Products)

Synonyms: DHRD Protein, DRAD Protein, FBLN3 Protein, FBNL Protein, FIBL-3 Protein, MLVT Protein, MTLV Protein, S1-5 Protein, T16 Protein, EGF containing fibulin like extracellular matrix protein 1 Protein, EGF-containing fibulin-like extracellular matrix protein 1 Protein, epidermal growth factor-containing fibulin-like extracellular matrix protein 1 Protein, EFEMP1 Protein, Efemp1 Protein

Background: This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]

Molecular Weight: 52.7 kDa

NCBI Accession: NP_004096

Pathways: EGFR Signaling Pathway