PHD1 Protein (His tag)
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- Target See all PHD1 (EGLN2) Proteins
- PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PHD1 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human EGLN2 / PHD1 (N-term HIS tag, transcript variant 3) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product EGLN2 Protein
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Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (AA 1-407) protein (GST tag)
EGLN2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (AA 1-407) protein (Strep Tag)Crystallography grade EGLN2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (AA 1-419) protein (Strep Tag)Crystallography grade EGLN2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)EGLN2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (AA 283-407), (partial) protein (His tag)EGLN2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))
- Alternative Name
- Egln2,phd1 (EGLN2 Products)
- Synonyms
- egln2 Protein, phd2 Protein, PHD1 Protein, 0610011A13Rik Protein, C85656 Protein, Hif-p4h-1 Protein, Ier4 Protein, Phd1 Protein, SM-20 Protein, HIF-PH1 Protein, HPH-1 Protein, HPH-3 Protein, PHD-1 Protein, EIT6 Protein, HIFPH1 Protein, egl-9 family hypoxia-inducible factor 1 Protein, egl-9 family hypoxia inducible factor 2 Protein, Egl nine homolog 2 Protein, egl-9 family hypoxia-inducible factor 2 L homeolog Protein, egl-9 family hypoxia-inducible factor 2 Protein, egln1 Protein, EGLN2 Protein, egln2 Protein, egln2.L Protein, Egln2 Protein
- Background
- The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.
- Molecular Weight
- 43.5 kDa
- NCBI Accession
- NP_542770
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis
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