ERN1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2720502

Product Details

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ERN1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human ERN1 / IRE1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Lee, Wang, Reyes, Armstrong, Kulikowicz, Santos, Lee, Koehler, Martin: "Hypothermia and Rewarming Activate a Macroglial Unfolded Protein Response Independent of Hypoxic-Ischemic Brain Injury in Neonatal Piglets." in: Developmental neuroscience, Vol. 38, Issue 4, pp. 277-294, (2016) (PubMed).

Target Details

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Alternative Name: Ern1,ire1 (ERN1 Products)

Synonyms: IRE1 Protein, IRE1P Protein, IRE1a Protein, hIRE1p Protein, 9030414B18Rik Protein, AI225830 Protein, C85377 Protein, Ire1a Protein, Ire1alpha Protein, Ire1p Protein, RGD1559716 Protein, Ern1 Protein, Ire1 Protein, Ire1b Protein, sb:eu1005 Protein, zgc:109932 Protein, endoplasmic reticulum to nucleus signaling 1 Protein, endoplasmic reticulum to nucleus signaling 2 Protein, endoplasmic reticulum (ER) to nucleus signalling 1 Protein, serine/threonine-protein kinase/endoribonuclease IRE1 Protein, endoplasmic reticulum (ER) to nucleus signalling 2 Protein, ERN1 Protein, ERN2 Protein, Ern1 Protein, LOC524719 Protein, Ern2 Protein, ern1 Protein

Background: The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals.

Molecular Weight: 109.6 kDa

NCBI Accession: NP_001424

Pathways: ER-Nucleus Signaling, Unfolded Protein Response