EWSR1 Protein (Transcript Variant EWS) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2720598

Product Details

Target: EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant EWS

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This EWSR1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human EWSR1 (transcript variant EWS) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Altmeyer, Neelsen, Teloni, Pozdnyakova, Pellegrino, Grøfte, Rask, Streicher, Jungmichel, Nielsen, Lukas: "Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose)." in: Nature communications, Vol. 6, pp. 8088, (2015) (PubMed).

Target Details

Target: EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

Alternative Name: Ewsr1 (EWSR1 Products)

Synonyms: EWS Protein, bK984G1.4 Protein, AU018891 Protein, Ews Protein, Ewsh Protein, EWSR1 Protein, fc04c01 Protein, wu:fc04c01 Protein, DKFZp459K1116 Protein, ewsr1 Protein, ewsr1.S Protein, fb40b11 Protein, fusl Protein, wu:fb40b11 Protein, wu:fb75g09 Protein, zgc:55864 Protein, EWS RNA binding protein 1 Protein, Ewing sarcoma breakpoint region 1 Protein, EWS RNA-binding protein 1 Protein, EWS RNA-binding protein 1a Protein, EWS RNA binding protein 1 L homeolog Protein, EWS RNA-binding protein 1b Protein, EWSR1 Protein, Ewsr1 Protein, ewsr1a Protein, ewsr1 Protein, ewsr1.L Protein, ewsr1b Protein

Background: This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(1122)(q24q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.

Molecular Weight: 68.3 kDa

NCBI Accession: NP_005234