FGF2 Protein

Catalog No. ABIN2721053

Product Details

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human FGF basic / FGF2 protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: <0.1 EU per 1 μg of the protein by the LAL

Biological Activity Comment: Measured in a cell proliferation assay using 3T3 cells. ED50 for this effect is typically 0.07-0.10 ng/ml.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a sterile solution containing 10 mM Tris.Cl, pH 7.0, 150 mM NaCl. Stable for at least 6 months from date of receipt under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Ye, Ge, Zhang, Cheng, Zhang, He, Wang, Lin, Yang, Liu, Zhao, Deng: "Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds." in: Cell research, Vol. 26, Issue 1, pp. 34-45, (2016) (PubMed).

Li, Zuo, Jing, Ma, Wang, Liu, Zhu, Du, Xiong, Du, Xu, Xiao, Wang, Chai, Zhao, Deng: "Small-Molecule-Driven Direct Reprogramming of Mouse Fibroblasts into Functional Neurons." in: Cell stem cell, Vol. 17, Issue 2, pp. 195-203, (2015) (PubMed).

Hou, Li, Zhang, Liu, Guan, Li, Zhao, Ye, Yang, Liu, Ge, Xu, Zhang, Zhao, Deng: "Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds." in: Science (New York, N.Y.), Vol. 341, Issue 6146, pp. 651-4, (2013) (PubMed).

Target Details

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: Fgf Basic,fgf2 (FGF2 Products)

Synonyms: BFGF Protein, FGF-2 Protein, FGFB Protein, HBGF-2 Protein, Fgf-2 Protein, Fgfb Protein, bFGF Protein, fibroblast growth factor 2 Protein, fibroblast growth factor 2 (basic) Protein, FGF2 Protein, Fgf2 Protein, fgf2 Protein

Background: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

Molecular Weight: 17.4 kDa

NCBI Accession: NP_001997

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins