FUBP1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2721443

Product Details

Target: FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FUBP1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human FUBP1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Miro, Laaref, Rofidal, Lagrafeuille, Hem, Thorel, Méchin, Mamchaoui, Mouly, Claustres, Tuffery-Giraud: "FUBP1: a new protagonist in splicing regulation of the DMD gene." in: Nucleic acids research, Vol. 43, Issue 4, pp. 2378-89, (2015) (PubMed).

Target Details

Target: FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

Alternative Name: Fubp1 (FUBP1 Products)

Synonyms: FBP Protein, FUBP Protein, fbp Protein, fubp Protein, MGC53183 Protein, MGC75632 Protein, FUBP1 Protein, DKFZp468N0812 Protein, 9530027K12Rik Protein, D3Ertd330e Protein, Fubp Protein, Fubp4 Protein, MGC78835 Protein, wu:fa92h11 Protein, wu:fb24h11 Protein, wu:fc26f06 Protein, zgc:66046 Protein, far upstream element binding protein 1 Protein, far upstream element (FUSE) binding protein 1 S homeolog Protein, far upstream element (FUSE) binding protein 1 Protein, far upstream element binding protein 1 L homeolog Protein, FUBP1 Protein, fubp1.S Protein, fubp1 Protein, Fubp1 Protein, fubp1.L Protein

Background: The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants.

Molecular Weight: 67.4 kDa

NCBI Accession: NP_003893