ADD3 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- Target See all ADD3 Proteins
- ADD3 (Adducin 3 (Gamma) (ADD3))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ADD3 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Gamma-adducin (ADD3) (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product ADD3 Protein
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Adducin 3 (Gamma) (ADD3) (AA 462-560) protein (GST tag)
ADD3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Adducin 3 (Gamma) (ADD3) (AA 2-706) protein (His tag)Crystallography grade ADD3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Adducin 3 (Gamma) (ADD3) (AA 2-706) protein (His tag)Crystallography grade ADD3 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Adducin 3 (Gamma) (ADD3) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)ADD3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- ADD3 (Adducin 3 (Gamma) (ADD3))
- Alternative Name
- gamma-Adducin (Add3) (ADD3 Products)
- Synonyms
- gamma-adducin Protein, add3 Protein, MGC89418 Protein, ADD3 Protein, DKFZp459E0630 Protein, ADDL Protein, AI463285 Protein, R75380 Protein, adducin 3 (gamma) Protein, adducin 3 Protein, adducin 3 L homeolog Protein, ADD3 Protein, add3 Protein, add3.L Protein, Add3 Protein
- Background
- Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known.
- Molecular Weight
- 79 kDa
- NCBI Accession
- NP_058432
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