GCLC Protein (Myc-DYKDDDDK Tag)
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- Target See all GCLC Proteins
- GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GCLC protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human GCLC / GLCLC protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product GCLC Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
- Alternative Name
- Gclc,glclc (GCLC Products)
- Synonyms
- GCLC Protein, gclc Protein, MGC146287 Protein, 2259 Protein, CG2259 Protein, DmGCLC Protein, DmGCS Protein, DmGCSh Protein, Dmel\\CG2259 Protein, GCL Protein, GCLc Protein, GCSh Protein, Gcs Protein, Gcsh Protein, DDBDRAFT_0186120 Protein, DDBDRAFT_0231403 Protein, DDB_0186120 Protein, DDB_0231403 Protein, GCS Protein, GLCL Protein, GLCLC Protein, D9Wsu168e Protein, GLCL-H Protein, Ggcs-hs Protein, Glclc Protein, cb1049 Protein, fe36e11 Protein, wu:fe36e11 Protein, glutamate-cysteine ligase catalytic subunit Protein, glutamate-cysteine ligase, catalytic subunit Protein, Glutamate-cysteine ligase catalytic subunit Protein, gamma glutamylcysteine synthetase Protein, glutamate-cysteine ligase, catalytic subunit L homeolog Protein, glutamate-cysteine ligase, catalytic subunit S homeolog Protein, gamma-glutamylcysteine synthetase Protein, GCLC Protein, gclc Protein, Gclc Protein, LbGCS Protein, gcsA Protein, gclc.L Protein, gclc.S Protein, GSH1 Protein
- Background
- Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010].
- Molecular Weight
- 72.6 kDa
- NCBI Accession
- NP_001489
- Pathways
- Cell RedoxHomeostasis
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