GCSH Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2721771

Product Details

Target: GCSH (Glycine Cleavage System H Protein (GCSH))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This GCSH protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human GCSH protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: GCSH (Glycine Cleavage System H Protein (GCSH))

Alternative Name: Gcsh (GCSH Products)

Synonyms: GCE Protein, NKH Protein, GCSH Protein, H-PROTEIN Protein, 1100001L02Rik Protein, 5730591C18Rik Protein, wu:fb14d10 Protein, wu:fw84b08 Protein, zgc:112535 Protein, BA5228 Protein, gce Protein, gcsh Protein, nkh Protein, wu:fd56h06 Protein, zgc:110557 Protein, zgc:92732 Protein, glycine cleavage system protein H Protein, glycine cleavage system protein H (aminomethyl carrier) Protein, glycine cleavage system lipoyl carrier protein GcvH Protein, glycine cleavage system H protein Protein, glycine cleavage system protein H (aminomethyl carrier), a Protein, glycine cleavage system carrier of aminomethyl moiety GcvH Protein, leucine rich repeat containing 34 Protein, glycine cleavage system protein H (aminomethyl carrier) L homeolog Protein, glycine cleavage system protein H (aminomethyl carrier), b Protein, GCSH Protein, Gcsh Protein, gcvH-2 Protein, gcvH Protein, gcsha Protein, LRRC34 Protein, gcsh.L Protein, gcshb Protein

Background: Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Two transcript variants, one protein-coding and the other probably not protein-coding,have been found for this gene. Also, several transcribed and non-transcribed pseudogenes of this gene exist throughout the genome.[provided by RefSeq, Jan 2010].

Molecular Weight: 13.8 kDa

NCBI Accession: NP_004474