GDF15 Protein
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- Target See all GDF15 Proteins
- GDF15 (Growth Differentiation Factor 15 (GDF15))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- CHO Cells
- Application
- Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human GDF15 protein expressed in Cell Culture.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3/E1 osetoblast cells. The expected ED50 for this effect is 1.0-3.0 μg/ml
- Top Product
- Discover our top product GDF15 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 10 mM Sodium Citrate, pH 3.0
- Handling Advice
- Centrifuge the vial prior to opening. Reconstitute the protein in water to a concnetration of 0.1 ~ 1.0 mg/mL. Do not vertex. For extended storage, it is recommended to further dilute in a proper buffer containing a carrier protein such as 0.1 % BSA, and store in working aliquots at -20 °C or -80 °C
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- GDF15 (Growth Differentiation Factor 15 (GDF15))
- Alternative Name
- Gdf15 (GDF15 Products)
- Synonyms
- GDF15 Protein, GDF-15 Protein, MIC-1 Protein, MIC1 Protein, NAG-1 Protein, PDF Protein, PLAB Protein, PTGFB Protein, SBF Protein, growth differentiation factor 15 Protein, GDF15 Protein, Gdf15 Protein
- Background
- This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The protein is expressed in a broad range of cell types, acts as a pleiotropic cytokine and is involved in the stress response program of cells after cellular injury. Increased protein levels are associated with disease states such as tissue hypoxia, inflammation, acute injury and oxidative stress.
- Molecular Weight
- 12 kDa
- NCBI Accession
- NP_004855
- Pathways
- SARS-CoV-2 Protein Interactome
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