GEMIN5 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2721790

Product Details

Target: GEMIN5 (Gem (Nuclear Organelle) Associated Protein 5 (GEMIN5))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This GEMIN5 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Gemin-5 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Francisco-Velilla, Fernandez-Chamorro, Ramajo, Martinez-Salas: "The RNA-binding protein Gemin5 binds directly to the ribosome and regulates global translation." in: Nucleic acids research, Vol. 44, Issue 17, pp. 8335-51, (2016) (PubMed).

Target Details

Target: GEMIN5 (Gem (Nuclear Organelle) Associated Protein 5 (GEMIN5))

Alternative Name: Gemin-5 (GEMIN5 Products)

Synonyms: GEMIN-5 Protein, AA407055 Protein, AA407208 Protein, AI451603 Protein, BB194447 Protein, C330013N08 Protein, gem nuclear organelle associated protein 5 Protein, gem (nuclear organelle) associated protein 5 Protein, GEMIN5 Protein, Gemin5 Protein

Background: This gene encodes a WD repeat protein that is a component of the survival of motor neurons (SMN) complex. The SMN complex plays a critical role in mRNA splicing through the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), and may also mediate the assembly and transport of other classes of ribonucleoproteins. The encoded protein is the snRNA-binding component of the SMN complex. Dysregulation of this gene may play a role in alternative mRNA splicing and tumor cell motility. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 168.4 kDa

NCBI Accession: NP_056280

Pathways: Ribonucleoprotein Complex Subunit Organization