HSP70 1A Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2722555

Product Details

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This HSP70 1A protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human Heat shock 70 kDa protein 1A (HSPA1A) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: Higher specific activity than E. coli derived HSP70: human recombinant Hsp70 was compared side-by-side with E. coli derived Hsp70 in a firefly luciferase refolding assay. Percentage of refolding is relative to an identical load of non-denatured luciferase in the reaction. The human cell produced Hsp70 is approximately 30% more active than the bacterial produced Hsp70

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Abstract: HSPA1A Products

Synonyms: hsp70-4 Protein, hspa1a Protein, Hsp70-3 Protein, Hsp70.3 Protein, Hsp72 Protein, hsp68 Protein, hsp70A1 Protein, HSP72 Protein, Hsp70-1 Protein, Hspa1 Protein, Hspa1b Protein, hsp70-1 Protein, hsp70-1a Protein, hsp70i Protein, hsp72 Protein, hspa1 Protein, hspa1b Protein, HSP70-1 Protein, HSP70-1A Protein, HSP70I Protein, HSPA1 Protein, HSPA1A Protein, HSPA1B Protein, HSP70-2 Protein, HSPA2 Protein, HSP70 Protein, hspA1A Protein, heat shock cognate 70-kd protein, tandem duplicate 3 Protein, heat shock 70 kDa protein 1 Protein, heat shock 70 kDa protein II Protein, heat shock protein 1A Protein, heat shock 70kD protein 1A Protein, heat shock protein family A (Hsp70) member 1A S homeolog Protein, heat shock protein family A (Hsp70) member 1A Protein, heat shock protein 70.2 Protein, heat shock 70kDa protein 1A Protein, heat shock 70 kDa protein 1B Protein, heat shock protein 70.1 Protein, hsp70.3 Protein, LOC100023597 Protein, LOC100554002 Protein, LOC100608888 Protein, Hspa1a Protein, hspa1a.S Protein, HSPA1A Protein, HSP70.2 Protein, LOC100354037 Protein, HSP70.1 Protein

Background: This intronless gene encodes a 70 kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Molecular Weight: 69.9 kDa

NCBI Accession: NP_005336

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process