Histone H2B Type 1-C/E/F/G/I (LOC100407767) protein (His tag)

Catalog No. ABIN2722811

Product Details

Target: Histone H2B Type 1-C/E/F/G/I (LOC100407767)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: His tag

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Histone H2B type 1-C/E/F/G/I (full length, N-term HIS tag) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Histone H2B Type 1-C/E/F/G/I (LOC100407767)

Alternative Name: Histone h2b Type 1-C/e/f/g/i (LOC100407767 Products)

Synonyms: H2B.1 Protein, H2B/l Protein, H2BFL Protein, dJ221C16.3 Protein, histone cluster 1 H2B family member c Protein, HIST1H2BC Protein

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Molecular Weight: 13.7 kDa

NCBI Accession: NP_003514