Histone Cluster 3, H3 (HIST3H3) Protein
HIST3H3
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
1 image
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- Target See all Histone Cluster 3, H3 (HIST3H3) Proteins
- Histone Cluster 3, H3 (HIST3H3)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Histone H3.1t protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- <0.1 EU per 1 μg of the protein by the LAL
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a sterile solution containing 20 mM phosphate buffer, pH 7.4. Stable for at least 6 months from date of receipt under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Histone Cluster 3, H3 (HIST3H3)
- Alternative Name
- Histone h3.1t (HIST3H3 Products)
- Synonyms
- H3.4 Protein, H3/g Protein, H3FT Protein, H3t Protein, H3/A Protein, H3FA Protein, H3.2-221 Protein, Hist1 Protein, histone cluster 3 H3 Protein, histone cluster 1 H3 family member a Protein, histone cluster 3, H3 Protein, histone cluster 1, H3f Protein, HIST3H3 Protein, HIST1H3A Protein, Hist1h3f Protein
- Background
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
- Molecular Weight
- 15.5 kDa
- NCBI Accession
- NP_003484
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