HLA-DOB Protein (Myc-DYKDDDDK Tag)
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- Target See all HLA-DOB Proteins
- HLA-DOB (Major Histocompatibility Complex, Class II, DO beta (HLA-DOB))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HLA-DOB protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human HLA class II DO beta / HLA-DOB protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product HLA-DOB Protein
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Major Histocompatibility Complex, Class II, DO beta (HLA-DOB) (AA 27-116) protein (GST tag)
HLA-DOB Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Major Histocompatibility Complex, Class II, DO beta (HLA-DOB) (AA 27-224) protein (His tag)Crystallography grade HLA-DOB Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Major Histocompatibility Complex, Class II, DO beta (HLA-DOB) (AA 27-273) protein (rho-1D4 tag)Crystallography grade HLA-DOB Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- HLA-DOB (Major Histocompatibility Complex, Class II, DO beta (HLA-DOB))
- Alternative Name
- Hla Class II Do Beta,hla-Dob (HLA-DOB Products)
- Synonyms
- DOB Protein, major histocompatibility complex, class II, DO beta Protein, HLA-DOB Protein, PATR-DOB Protein
- Background
- HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.
- Molecular Weight
- 30.6 kDa
- NCBI Accession
- NP_002111
- Pathways
- Cancer Immune Checkpoints
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