HNRNPU Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2722939

Product Details

Target: HNRNPU (Heterogeneous Nuclear Ribonucleoprotein U (Scaffold Attachment Factor A) (HNRNPU))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 2

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This HNRNPU protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human hnRNP-U / HNRNPU (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: HNRNPU (Heterogeneous Nuclear Ribonucleoprotein U (Scaffold Attachment Factor A) (HNRNPU))

Alternative Name: Hnrnp-U,hnrnpu (HNRNPU Products)

Synonyms: AA408410 Protein, AI256620 Protein, AL024194 Protein, AL024437 Protein, AW557595 Protein, C86794 Protein, Hnrpu Protein, SAFA Protein, Sp120 Protein, hnRNP U Protein, SN1 Protein, HNRPU Protein, SAF-A Protein, U21.1 Protein, saf-a Protein, heterogeneous nuclear ribonucleoprotein U Protein, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) S homeolog Protein, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) Protein, Hnrnpu Protein, HNRNPU Protein, hnrnpu.S Protein

Background: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they form complexes with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene contains a RNA binding domain and scaffold-associated region (SAR)-specific bipartite DNA-binding domain. This protein is also thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis, this protein is cleaved in a caspase-dependent way. Cleavage occurs at the SALD site, resulting in a loss of DNA-binding activity and a concomitant detachment of this protein from nuclear structural sites. But this cleavage does not affect the function of the encoded protein in RNA metabolism. At least two alternatively spliced transcript variants have been identified for this gene.

Molecular Weight: 88.8 kDa

NCBI Accession: NP_004492