HUS1 Protein (Myc-DYKDDDDK Tag)
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- Target See all HUS1 Proteins
- HUS1 (HUS1 Checkpoint Homolog (S. Pombe) (HUS1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HUS1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human HUS1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product HUS1 Protein
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HUS1 Checkpoint Homolog (S. Pombe) (HUS1) (AA 1-280) protein (His tag)
HUS1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
HUS1 Checkpoint Homolog (S. Pombe) (HUS1) (AA 1-280) protein (GST tag)HUS1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
HUS1 Checkpoint Homolog (S. Pombe) (HUS1) (AA 1-280) protein (Strep Tag)Crystallography grade HUS1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
HUS1 Checkpoint Homolog (S. Pombe) (HUS1) (AA 1-280) protein (Strep Tag)Crystallography grade HUS1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
HUS1 Checkpoint Homolog (S. Pombe) (HUS1) (AA 2-280), (partial) protein (His-SUMO Tag)HUS1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- HUS1 (HUS1 Checkpoint Homolog (S. Pombe) (HUS1))
- Alternative Name
- Hus1 (HUS1 Products)
- Synonyms
- hHUS1 Protein, mHus1 Protein, XHus1 Protein, hus1 Protein, HUS1 checkpoint clamp component Protein, checkpoint clamp complex protein Hus1 Protein, HUS1 checkpoint clamp component L homeolog Protein, HUS1 Protein, Hus1 Protein, hus1 Protein, hus1.L Protein
- Background
- The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.
- Molecular Weight
- 31.5 kDa
- NCBI Accession
- NP_004498
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