ID3 Protein (Myc-DYKDDDDK Tag)
(1 reference)-
- Target See all ID3 Proteins
- ID3 (Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ID3 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
-
- Recombinant human ID3 / BHLHB25 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product ID3 Protein
-
Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) (AA 1-119) protein (GST tag)
ID3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) (AA 1-83) protein (GST tag)ID3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) (AA 1-119) protein (Strep Tag)Crystallography grade ID3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) (AA 1-119) protein (Strep Tag)Crystallography grade ID3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
-
The tag is located at the C-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
-
: "PCB153-induced overexpression of ID3 contributes to the development of microvascular lesions." in: PLoS ONE, Vol. 9, Issue 8, pp. e104159, (2014) (PubMed).
-
: "PCB153-induced overexpression of ID3 contributes to the development of microvascular lesions." in: PLoS ONE, Vol. 9, Issue 8, pp. e104159, (2014) (PubMed).
-
- Target
- ID3 (Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3))
- Alternative Name
- Id3,bhlhb25 (ID3 Products)
- Synonyms
- HEIR-1 Protein, bHLHb25 Protein, Hlh462 Protein, Idb3 Protein, fa56g03 Protein, tdsubc_2g3 Protein, wu:fa56g03 Protein, ik:tdsubc_2g3 Protein, xx:tdsubc_2g3 Protein, XId3 Protein, XIdI Protein, XIdIa Protein, XIdIb Protein, XIdx Protein, heir-1 Protein, id3 Protein, xid3 Protein, xidia Protein, ID3 Protein, XIdIIa Protein, inhibitor of DNA binding 3, HLH protein Protein, inhibitor of DNA binding 3 Protein, inhibitor of DNA binding 3, HLH protein L homeolog Protein, inhibitor of DNA binding 3, HLH protein S homeolog Protein, ID3 Protein, Id3 Protein, id3 Protein, id3.L Protein, id3.S Protein
- Background
- The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with other HLH proteins. However, the encoded protein lacks a basic DNA-binding domain and therefore inhibits the DNA binding of any HLH protein with which it interacts.
- Molecular Weight
- 12.8 kDa
- NCBI Accession
- NP_002158
-
