IDH2 Protein (DYKDDDDK Tag)
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- Target See all IDH2 Proteins
- IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This IDH2 protein is labelled with DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Isocitrate dehydrogenase / IDH2 (C-term DDK tag) protein expressed in Sf9 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product IDH2 Protein
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Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2) (AA 354-451) protein (GST tag)
IDH2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2) (AA 1-452) protein (GST tag)IDH2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2) (AA 40-452) protein (His tag)Crystallography grade IDH2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2) (AA 40-452) protein (His tag)Crystallography grade IDH2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2) protein (Myc-DYKDDDDK Tag)IDH2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))
- Alternative Name
- Isocitrate Dehydrogenase,idh2 (IDH2 Products)
- Background
- Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.
- Molecular Weight
- 46.6 kDa
- NCBI Accession
- NP_002159
- Pathways
- Warburg Effect
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