IDH3G Protein (His tag)

Catalog No. ABIN2723871

Product Details

Target: IDH3G (Isocitrate Dehydrogenase 3 (NAD+) gamma (IDH3G))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This IDH3G protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Isocitric dehydrogenase gamma / IDH3G (full length, N-term HIS tag, transcript variant 1) protein expressed in E.coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: IDH3G (Isocitrate Dehydrogenase 3 (NAD+) gamma (IDH3G))

Alternative Name: Isocitric Dehydrogenase Gamma,idh3g (IDH3G Products)

Synonyms: H-IDHG Protein, MGC83400 Protein, wu:fa01b02 Protein, wu:fc88f06 Protein, h-idhg Protein, IDH Protein, isocitrate dehydrogenase 3 (NAD(+)) gamma Protein, isocitrate dehydrogenase 3 (NAD+) gamma S homeolog Protein, isocitrate dehydrogenase 3 (NAD+) gamma Protein, isocitrate dehydrogenase 3 (NAD+), gamma Protein, isocitrate dehydrogenase 3 (NAD), gamma Protein, IDH3G Protein, idh3g.S Protein, idh3g Protein, Idh3g Protein

Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined.

Molecular Weight: 38.8 kDa

NCBI Accession: NP_004126