KPNA1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- Target See all KPNA1 Proteins
- KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This KPNA1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human KPNA1 / Importin alpha-1 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product KPNA1 Protein
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Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (AA 1-538) protein (GST tag)
KPNA1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (AA 1-538) protein (Strep Tag)Crystallography grade KPNA1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (AA 2-538) protein (His tag)Crystallography grade KPNA1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (AA 1-538) protein (Strep Tag)Crystallography grade KPNA1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (AA 2-538) protein (His tag)Crystallography grade KPNA1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))
- Alternative Name
- Kpna1,importin alpha-1 (KPNA1 Products)
- Synonyms
- IPOA5 Protein, NPI-1 Protein, RCH2 Protein, SRP1 Protein, AW494490 Protein, NPI1 Protein, Rch2 Protein, mSRP1 Protein, si:ch211-161h7.7 Protein, karyopherin subunit alpha 1 Protein, importin alpha-1 subunit Protein, karyopherin (importin) alpha 1 Protein, karyopherin alpha 1 (importin alpha 5) Protein, KPNA1 Protein, Kpna1 Protein, EHI_124900 Protein, kpna1 Protein
- Background
- Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 60 kDa
- NCBI Accession
- NP_002255
- Pathways
- M Phase, Protein targeting to Nucleus
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