LDHB Protein (Transcript Variant 2) (His tag)
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- Target See all LDHB Proteins
- LDHB (Lactate Dehydrogenase B (LDHB))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 2
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This LDHB protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human LDHB (transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- < 0.1 EU per μg protein as determined by LAL test
- Top Product
- Discover our top product LDHB Protein
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Lactate Dehydrogenase B (LDHB) (AA 1-334) protein (GST tag)
LDHB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Lactate Dehydrogenase B (LDHB) (AA 1-334) (Active) protein (His tag)LDHB Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
Lactate Dehydrogenase B (LDHB) (AA 2-334) protein (His tag)Crystallography grade LDHB Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Lactate Dehydrogenase B (LDHB) (AA 2-334) protein (His tag)Crystallography grade LDHB Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Lactate Dehydrogenase B (LDHB) (AA 2-334), (full length) protein (His tag)LDHB Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 20 mM Tris-HCl, 100 mM NaCl, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- LDHB (Lactate Dehydrogenase B (LDHB))
- Alternative Name
- Ldhb (LDHB Products)
- Synonyms
- LDH-B Protein, LDH-H Protein, LDHBD Protein, TRG-5 Protein, AI790582 Protein, H-Ldh Protein, Ldh-2 Protein, Ldh2 Protein, ldh-h Protein, ldh2 Protein, ldhb Protein, ldhba Protein, trg-5 Protein, LDHB Protein, LDH-B-A Protein, wu:fa16d07 Protein, wu:fa20c10 Protein, wu:fa96a08 Protein, LDH-B-B Protein, zgc:92882 Protein, lactate dehydrogenase B Protein, lactate dehydrogenase B S homeolog Protein, lactate dehydrogenase Ba Protein, lactate dehydrogenase Bb Protein, LDHB Protein, Ldhb Protein, ldhb.S Protein, ldhb Protein, ldhba Protein, ldhbb Protein
- Background
- This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.
- Molecular Weight
- 38.8 kDa
- NCBI Accession
- NP_001167568
- Pathways
- Warburg Effect
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