LIMK2 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2724748

Product Details

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This LIMK2 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human LIMK2 (transcript variant 2b) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Alternative Name: Limk2 (LIMK2 Products)

Synonyms: xlimk2 Protein, zgc:91990 Protein, wu:fa97c09 Protein, wu:fk73e11 Protein, LIMK2 Protein, Limk2b Protein, Link2 Protein, LIMK Protein, 9430059K01 Protein, A930024P04Rik Protein, C85310 Protein, Limk2a Protein, LIM domain kinase 2 L homeolog Protein, LIM domain kinase 2 Protein, LIM motif-containing protein kinase 2 Protein, limk2.L Protein, limk2 Protein, LIMK2 Protein, Limk2 Protein

Background: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 69.7 kDa

NCBI Accession: NP_057952