MAN2B1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2725053

Product Details

Target: MAN2B1 (Mannosidase, Alpha, Class 2B, Member 1 (MAN2B1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This MAN2B1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Lysosomal Alpha-Mannosidase protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: MAN2B1 (Mannosidase, Alpha, Class 2B, Member 1 (MAN2B1))

Alternative Name: Lysosomal alpha-Mannosidase (MAN2B1 Products)

Synonyms: AW107687 Protein, LAMAN Protein, Laman Protein, Manb Protein, MANB Protein, mannosidase alpha class 2B member 1 Protein, mannosidase, alpha, class 2B, member 1 Protein, mannosidase 2, alpha B1 Protein, MAN2B1 Protein, man2b1 Protein, Man2b1 Protein

Background: This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010].

Molecular Weight: 108.7 kDa

NCBI Accession: NP_000519