MAN2B1 Protein (Myc-DYKDDDDK Tag)
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- Target See all MAN2B1 Proteins
- MAN2B1 (Mannosidase, Alpha, Class 2B, Member 1 (MAN2B1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MAN2B1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Lysosomal Alpha-Mannosidase protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product MAN2B1 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- MAN2B1 (Mannosidase, Alpha, Class 2B, Member 1 (MAN2B1))
- Alternative Name
- Lysosomal alpha-Mannosidase (MAN2B1 Products)
- Synonyms
- AW107687 Protein, LAMAN Protein, Laman Protein, Manb Protein, MANB Protein, mannosidase alpha class 2B member 1 Protein, mannosidase, alpha, class 2B, member 1 Protein, mannosidase 2, alpha B1 Protein, MAN2B1 Protein, man2b1 Protein, Man2b1 Protein
- Background
- This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010].
- Molecular Weight
- 108.7 kDa
- NCBI Accession
- NP_000519
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