MARCH10 Protein (His tag)
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- Target See all MARCH10 Proteins
- MARCH10 (Membrane-Associated Ring Finger (C3HC4) 10, E3 Ubiquitin Protein Ligase (MARCH10))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MARCH10 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human MARCH10 / RNF190 (full length, N-term HIS tag, transcript variant 1) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product MARCH10 Protein
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Membrane-Associated Ring Finger (C3HC4) 10, E3 Ubiquitin Protein Ligase (MARCH10) (AA 76-184) protein (GST tag)
MARCH10 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Membrane-Associated Ring Finger (C3HC4) 10, E3 Ubiquitin Protein Ligase (MARCH10) (AA 1-657) protein (GST tag)MARCH10 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Membrane-Associated Ring Finger (C3HC4) 10, E3 Ubiquitin Protein Ligase (MARCH10) (AA 1-808) protein (His tag)Crystallography grade MARCH10 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- MARCH10 (Membrane-Associated Ring Finger (C3HC4) 10, E3 Ubiquitin Protein Ligase (MARCH10))
- Alternative Name
- March10,rnf190 (MARCH10 Products)
- Synonyms
- 4933417C16Rik Protein, AV039223 Protein, OTTMUSG00000002847 Protein, Rnf190 Protein, MARCH-X Protein, RGD1311692 Protein, RNF190 Protein, membrane associated ring-CH-type finger 10 Protein, membrane-associated ring finger (C3HC4) 10 Protein, MARCH10 Protein, March10 Protein
- Background
- MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010]
- Molecular Weight
- 90.3 kDa
- NCBI Accession
- NP_689811
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