MARCH2 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- Target See all MARCH2 Proteins
- MARCH2 (Membrane-Associated Ring Finger (C3HC4) 2, E3 Ubiquitin Protein Ligase (MARCH2))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MARCH2 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human MARCH2 / RNF172 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product MARCH2 Protein
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Membrane-Associated Ring Finger (C3HC4) 2, E3 Ubiquitin Protein Ligase (MARCH2) (AA 1-176) protein (GST tag)
MARCH2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Membrane-Associated Ring Finger (C3HC4) 2, E3 Ubiquitin Protein Ligase (MARCH2) (AA 2-99) protein (GST tag)MARCH2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Membrane-Associated Ring Finger (C3HC4) 2, E3 Ubiquitin Protein Ligase (MARCH2) (AA 1-246) protein (rho-1D4 tag)Crystallography grade MARCH2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Membrane-Associated Ring Finger (C3HC4) 2, E3 Ubiquitin Protein Ligase (MARCH2) (AA 1-246) protein (rho-1D4 tag)Crystallography grade MARCH2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Membrane-Associated Ring Finger (C3HC4) 2, E3 Ubiquitin Protein Ligase (MARCH2) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)MARCH2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- MARCH2 (Membrane-Associated Ring Finger (C3HC4) 2, E3 Ubiquitin Protein Ligase (MARCH2))
- Alternative Name
- March2,rnf172 (MARCH2 Products)
- Synonyms
- MARCH-II Protein, RNF172 Protein, rgd1306395 Protein, marh2 Protein, RGD1306395 Protein, 9530046H09Rik Protein, membrane associated ring-CH-type finger 2 Protein, membrane associated ring-CH-type finger 2 S homeolog Protein, E3 ubiquitin-protein ligase MARCH2 Protein, membrane-associated ring finger (C3HC4) 2 Protein, MARCH2 Protein, march2.S Protein, march2 Protein, Tsp_09446 Protein, marh2 Protein, March2 Protein
- Background
- MARCH2 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH2 reduces surface accumulation of several glycoproteins and appears to regulate early endosome-to-trans-Golgi network (TGN) trafficking (Bartee et al., 2004 [PubMed 14722266] Nakamura et al., 2005 [PubMed 15689499]).[supplied by OMIM, Mar 2010]
- Molecular Weight
- 26.8 kDa
- NCBI Accession
- NP_057580
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