MARCH8 Protein (Transcript Variant 7) (Myc-DYKDDDDK Tag)
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- Target See all MARCH8 Proteins
- MARCH8 (Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 7
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MARCH8 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human MARCH8 (transcript variant 7) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product MARCH8 Protein
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Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8) (AA 1-291) protein (GST tag)
MARCH8 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8) (AA 77-153) protein (GST tag)MARCH8 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8) (AA 1-291) protein (rho-1D4 tag)Crystallography grade MARCH8 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8) (AA 1-286) protein (rho-1D4 tag)Crystallography grade MARCH8 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8) (Transcript Variant 8) protein (Myc-DYKDDDDK Tag)MARCH8 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8) (Transcript Variant 6) protein (Myc-DYKDDDDK Tag)MARCH8 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- MARCH8 (Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8))
- Alternative Name
- March8 (MARCH8 Products)
- Synonyms
- MARCH-VIII Protein, MIR Protein, RNF178 Protein, c-MIR Protein, zgc:171495 Protein, 1300017E09Rik Protein, Mir Protein, Mar-08 Protein, MARCH8 Protein, membrane associated ring-CH-type finger 8 Protein, membrane-associated ring finger (C3HC4) 8 Protein, membrane associated ring-CH-type finger 8 L homeolog Protein, MARCH8 Protein, march8 Protein, March8 Protein, march8.L Protein
- Background
- MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153] Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]
- Molecular Weight
- 32.8 kDa
- NCBI Accession
- NP_001002266
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