MRPL4 Protein (His tag)
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- Target See all MRPL4 Proteins
- MRPL4 (Mitochondrial Ribosomal Protein L4 (MRPL4))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MRPL4 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human MRPL4 (full length, N-term HIS tag, transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product MRPL4 Protein
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Mitochondrial Ribosomal Protein L4 (MRPL4) (AA 1-263) protein (GST tag)
MRPL4 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Mitochondrial Ribosomal Protein L4 (MRPL4) (AA 1-294) protein (Strep Tag)Crystallography grade MRPL4 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Mitochondrial Ribosomal Protein L4 (MRPL4) (AA 1-311) protein (Strep Tag)Crystallography grade MRPL4 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- MRPL4 (Mitochondrial Ribosomal Protein L4 (MRPL4))
- Alternative Name
- Mrpl4 (MRPL4 Products)
- Synonyms
- CGI-28 Protein, L4mt Protein, 1110017G11Rik Protein, mrpl4 Protein, zgc:110034 Protein, mitochondrial ribosomal protein L4 Protein, MRPL4 Protein, Mrpl4 Protein, mrpl4 Protein
- Background
- Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms.
- Molecular Weight
- 34.7 kDa
- NCBI Accession
- NP_057040
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