MYLK Protein (Transcript Variant 7) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2726683

Product Details

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 7

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This MYLK protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human MYLK (transcript variant 7) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Alternative Name: Mylk (MYLK Products)

Synonyms: AAT7 Protein, KRP Protein, MLCK Protein, MLCK1 Protein, MLCK108 Protein, MLCK210 Protein, MSTP083 Protein, MYLK1 Protein, smMLCK Protein, 9530072E15Rik Protein, A930019C19Rik Protein, AW489456 Protein, Mlck Protein, SMMLCK Protein, mlck Protein, fj60a06 Protein, mlck1a Protein, mylk Protein, wu:fj60a06 Protein, zmlck1 Protein, myosin light chain kinase Protein, myosin, light polypeptide kinase Protein, myosin, light chain kinase a Protein, MYLK Protein, Mylk Protein, mylka Protein

Background: This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.

Molecular Weight: 16.7 kDa

NCBI Accession: NP_444259