ME1 Protein (Myc-DYKDDDDK Tag)
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- Target See all ME1 Proteins
- ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ME1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human NADP-ME protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product ME1 Protein
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Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 226-572) protein (His tag)
ME1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-572) protein (GST tag)ME1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-572) protein (Strep Tag)Crystallography grade ME1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-572) protein (Strep Tag)Crystallography grade ME1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))
- Alternative Name
- Nadp-Me (ME1 Products)
- Synonyms
- HUMNDME Protein, MES Protein, D9Ertd267e Protein, Mdh-1 Protein, Mod-1 Protein, Mod1 Protein, MOD1 Protein, ME1 Protein, zgc:153079 Protein, malic enzyme 1 Protein, malic enzyme 1, NADP(+)-dependent, cytosolic Protein, ME1 Protein, Me1 Protein, me1 Protein
- Background
- This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
- Molecular Weight
- 64 kDa
- NCBI Accession
- NP_002386
- Pathways
- Regulation of Lipid Metabolism by PPARalpha
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