NCF4 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- Target See all NCF4 Proteins
- NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This NCF4 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human NCF-4 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product NCF4 Protein
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Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 1-339) protein (His tag)
NCF4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 1-339) protein (GST tag)NCF4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 1-100) protein (GST tag)NCF4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 1-339) protein (Strep Tag)Crystallography grade NCF4 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))
- Alternative Name
- Ncf-4 (NCF4 Products)
- Synonyms
- p40phox Protein, AI451400 Protein, NCF Protein, P40PHOX Protein, SH3PXD4 Protein, neutrophil cytosolic factor 4 Protein, ncf4 Protein, Ncf4 Protein, NCF4 Protein
- Background
- The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
- Molecular Weight
- 38.9 kDa
- NCBI Accession
- NP_000622
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