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HECW2 Protein (Myc-DYKDDDDK Tag)

HECW2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Catalog No. ABIN2727068
  • Target See all HECW2 Proteins
    HECW2 (HECT, C2 and WW Domain Containing E3 Ubiquitin Protein Ligase 2 (HECW2))
    Protein Type
    Recombinant
    Origin
    • 2
    • 1
    Human
    Source
    • 2
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This HECW2 protein is labelled with Myc-DYKDDDDK Tag.
    Application
    Antibody Production (AbP), Standard (STD)
    Characteristics
    • Recombinant human NEDL2 protein expressed in HEK293 cells.
    • Produced with end-sequenced ORF clone
    Purity
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
    Top Product
    Discover our top product HECW2 Protein
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Comment

    The tag is located at the C-terminal.

    Restrictions
    For Research Use only
  • Concentration
    50 μg/mL
    Buffer
    25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    HECW2 (HECT, C2 and WW Domain Containing E3 Ubiquitin Protein Ligase 2 (HECW2))
    Alternative Name
    Nedl2 (HECW2 Products)
    Synonyms
    HECW2 Protein, NEDL2 Protein, A730039N16Rik Protein, D030049F17Rik Protein, Gm971 Protein, Nedl2 Protein, mKIAA1301 Protein, HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 Protein, E3 ubiquitin-protein ligase HECW2 Protein, HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2b Protein, Hecw2 Protein, HECW2 Protein, LOC531691 Protein, hecw2b Protein, hecw2 Protein
    Background
    This gene encodes a member of a family of E3 ubiquitin ligases which plays an important role in the proliferation, migration and differentiation of neural crest cells as a regulator of glial cell line-derived neurotrophic factor (GDNF)/Ret signaling. This gene also plays an important role in angiogenesis through stabilization of endothelial cell-to-cell junctions as a regulator of angiomotin-like 1 stability. The encoded protein contains an N-terminal calcium/lipid-binding (C2) domain involved in membrane targeting, two-four WW domains responsible for cellular localization and substrate recognition, and a C-terminal homologous with E6-associated protein C-terminus (HECT) catalytic domain. Naturally occurring mutations in this gene are associated with neurodevelopmental delay, hypotonia, and epilepsy. The decreased expression of this gene in the aganglionic colon is associated with Hirschsprung&aposs disease. Alternative splicing results in multiple transcript variants.
    Molecular Weight
    175.6 kDa
    NCBI Accession
    NP_065811
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