COBRA1 Protein (Myc-DYKDDDDK Tag)
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- Target See all COBRA1 Proteins
- COBRA1 (Cofactor of BRCA1 (COBRA1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This COBRA1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human NELF-B protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product COBRA1 Protein
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Cofactor of BRCA1 (COBRA1) (AA 1-475) protein (GST tag)
COBRA1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Cofactor of BRCA1 (COBRA1) (AA 1-580) protein (Strep Tag)Crystallography grade COBRA1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Cofactor of BRCA1 (COBRA1) (AA 1-580) protein (Strep Tag)Crystallography grade COBRA1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Cofactor of BRCA1 (COBRA1) (AA 8-199), (partial) protein (GST tag)COBRA1 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- COBRA1 (Cofactor of BRCA1 (COBRA1))
- Alternative Name
- Nelf-B (COBRA1 Products)
- Synonyms
- LOC100219583 Protein, COBRA1 Protein, NELF-B Protein, bZ1O1.4 Protein, cobra1 Protein, cobra2 Protein, fe01e12 Protein, wu:fe01e12 Protein, zgc:55628 Protein, MGC131073 Protein, Cobra1 Protein, RGD1307832 Protein, A730008L03Rik Protein, AB041607 Protein, AI663983 Protein, Nelf-b Protein, cofactor of BRCA1 Protein, negative elongation factor complex member B Protein, negative elongation factor complex member B L homeolog Protein, LOC100219583 Protein, NELFB Protein, nelfb Protein, nelfb.L Protein, Nelfb Protein
- Background
- NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2 MIM 606026), either NELFC or NELFD (TH1L MIM 605297), and NELFE (RDBP MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1 MIM 603555) and SPT5 (SUPT5H MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]
- Molecular Weight
- 65.5 kDa
- NCBI Accession
- NP_056271
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