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N-Glycanase 1 Protein (NGLY1) (Transcript Variant 1) (Myc-DYKDDDDK Tag)

NGLY1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Catalog No. ABIN2727246
  • Target See all N-Glycanase 1 (NGLY1) Proteins
    N-Glycanase 1 (NGLY1)
    Protein Type
    Recombinant
    Protein Characteristics
    Transcript Variant 1
    Origin
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 5
    • 2
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This N-Glycanase 1 protein is labelled with Myc-DYKDDDDK Tag.
    Application
    Antibody Production (AbP), Standard (STD)
    Characteristics
    • Recombinant human NGLY1 (transcript variant 1) protein expressed in HEK293 cells.
    • Produced with end-sequenced ORF clone
    Purity
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
    Top Product
    Discover our top product NGLY1 Protein
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Comment

    The tag is located at the C-terminal.

    Restrictions
    For Research Use only
  • Concentration
    50 μg/mL
    Buffer
    25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    N-Glycanase 1 (NGLY1)
    Alternative Name
    Ngly1 (NGLY1 Products)
    Synonyms
    CDG1V Protein, PNG1 Protein, PNGase Protein, 1110002C09Rik Protein, Png1 Protein, im:6895151 Protein, zgc:110561 Protein, AtPNG1 Protein, K6M13.12 Protein, K6M13_12 Protein, peptide-N-glycanase 1 Protein, N-glycanase 1 Protein, N-glycanase 1 S homeolog Protein, peptide-N-glycanase 1 Protein, Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase Protein, similar to S. cerevisiae PNG1 (YPL096W) peptide:N-glycanase de-N-glycosylation enzyme Protein, NGLY1 Protein, ngly1.S Protein, ngly1 Protein, Ngly1 Protein, PNG1 Protein, png-1 Protein
    Background
    This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009].
    Molecular Weight
    74.2 kDa
    NCBI Accession
    NP_060767
    Pathways
    Cell RedoxHomeostasis, SARS-CoV-2 Protein Interactome
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