Nischarin Protein (NISCH) (Myc-DYKDDDDK Tag)
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- Target See all Nischarin (NISCH) Proteins
- Nischarin (NISCH)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Nischarin protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Nischarin protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product NISCH Protein
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Nischarin (NISCH) (AA 1246-1345) protein (GST tag)
NISCH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Nischarin (NISCH) (AA 1-1593) protein (Strep Tag)Crystallography grade NISCH Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Nischarin (NISCH) (AA 2-1504) protein (His tag)Crystallography grade NISCH Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Nischarin (NISCH)
- Alternative Name
- Nischarin (NISCH Products)
- Synonyms
- NISCH Protein, IRAS Protein, 1200007D05Rik Protein, 3202002H23Rik Protein, AW494485 Protein, I-1 Protein, IR1 Protein, hIRAS Protein, nischarin Protein, nischarin S homeolog Protein, NISCH Protein, Cyan7822_6841 Protein, nisch.S Protein, nisch Protein, Nisch Protein
- Background
- This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor.
- Molecular Weight
- 166.5 kDa
- NCBI Accession
- NP_009115
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