NUCB2 Protein
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- Target See all NUCB2 Proteins
- NUCB2 (Nucleobindin 2 (NUCB2))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human Nucleobindin-2 protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by an in vivo assay using healthy wild type male mice (C57BL/6J). Mice were treated via intraperitoneal injection once at a dose of 4&mug Nesfatin-1/gm of body weight. Significant effects on body weight and food consumption were observed relative to saline-treated controls.
- Top Product
- Discover our top product NUCB2 Protein
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Nucleobindin 2 (NUCB2) (AA 25-106) protein (His tag)
NUCB2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Nucleobindin 2 (NUCB2) (AA 25-106) protein (His tag)NUCB2 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Nucleobindin 2 (NUCB2) (AA 322-420) protein (GST tag)NUCB2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Nucleobindin 2 (NUCB2) (AA 25-420) protein (His tag)Crystallography grade NUCB2 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Nucleobindin 2 (NUCB2) (AA 25-106) protein (His tag)Crystallography grade NUCB2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- NUCB2 (Nucleobindin 2 (NUCB2))
- Alternative Name
- Nucleobindin-2 (NUCB2 Products)
- Synonyms
- nefa Protein, MGC97715 Protein, NUCB2 Protein, nucleobindin-2 Protein, NEFA Protein, AI607786 Protein, Calnuc Protein, Nefa Protein, Nesfatin-1 Protein, p54 Protein, nucleobindin 2 Protein, nucleobindin 2 L homeolog Protein, nucb2 Protein, NUCB2 Protein, Nucb2 Protein, nucb2.L Protein
- Background
- This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains.
- Molecular Weight
- 9.7 kDa
- NCBI Accession
- NP_005004
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