NUF2 Protein (His tag)

Catalog No. ABIN2727781

Product Details

Target: NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NUF2 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human NUF2 / CDCA1 (full length, N-term HIS tag, transcript variant 1) protein expressed in E.coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))

Alternative Name: Nuf2,cdca1 (NUF2 Products)

Synonyms: 2410003C07Rik Protein, AU044112 Protein, C85691 Protein, Cdca1 Protein, NUF2R Protein, CDCA1 Protein, CT106 Protein, zgc:56708 Protein, cdca1 Protein, ct106 Protein, nuf2 Protein, nuf2-a Protein, nuf2-b Protein, nuf2r Protein, xNuf2 Protein, cdca1-B Protein, xnuf2 Protein, NUF2, NDC80 kinetochore complex component Protein, NUF2, NDC80 kinetochore complex component, homolog Protein, spindle pole body protein Nuf2 Protein, NUF2, NDC80 kinetochore complex component L homeolog Protein, NUF2, NDC80 kinetochore complex component S homeolog Protein, Nuf2 Protein, NUF2 Protein, nuf2 Protein, nuf2.L Protein, nuf2.S Protein

Background: This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.

Molecular Weight: 54.1 kDa

NCBI Accession: NP_663735

Pathways: Maintenance of Protein Location