alpha Ketoglutarate Dehydrogenase (alphaKGDHC) protein (His tag)

Details for Product No. ABIN2727878, Supplier: Log in to see
Protein Name
  • AKGDH
  • E1k
  • OGDC
  • 2210403E04Rik
  • 2210412K19Rik
  • AA409584
  • d1401
  • mKIAA4192
  • oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
  • OGDH
  • Ogdh
Origin
Human
3
1
1
Source
Escherichia coli (E. coli)
2
1
1
1
Protein Type
Recombinant
Purification tag / Conjugate
His tag
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
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Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human OGDH (N-term HIS tag, transcript variant 2) protein expressed in E. coli.
  • Produced with end-sequenced ORF clone
Purity > 80 % as determined by SDS-PAGE and Coomassie blue staining
Background This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009].
Molecular Weight 43.5 kDa
NCBI Accession NP_001003941
Research Area Signaling, Metabolism
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the N-terminal.

Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Supplier Images
Western Blotting (WB) image for alpha Ketoglutarate Dehydrogenase (alphaKGDHC) protein (His tag) (ABIN2727878) Validation with Western Blot
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