P4HA1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
-
- Target See all P4HA1 Proteins
- P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This P4HA1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
-
- Recombinant human P4HA1 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product P4HA1 Protein
-
Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1) (AA 340-534) protein (His-IF2DI Tag)
P4HA1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1) (AA 1-534) protein (GST tag)P4HA1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1) (AA 18-534) protein (His tag)Crystallography grade P4HA1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1) (AA 18-534) protein (His tag)Crystallography grade P4HA1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)P4HA1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
-
The tag is located at the C-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))
- Alternative Name
- p4ha1 (P4HA1 Products)
- Synonyms
- P4HA Protein, AL022634 Protein, P4ha Protein, PHalphaI Protein, prolyl 4-hydroxylase subunit alpha-1 Protein, prolyl 4-hydroxylase subunit alpha 1 Protein, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide Protein, CpipJ_CPIJ000975 Protein, CpipJ_CPIJ017131 Protein, Tsp_03346 Protein, Tsp_03347 Protein, Tsp_15957 Protein, P4HA1 Protein, P4ha1 Protein
- Background
- This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
- Molecular Weight
- 59 kDa
- NCBI Accession
- NP_000908
-
