P4HA3 Protein (His tag)
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- Target See all P4HA3 Proteins
- P4HA3 (Prolyl 4-Hydroxylase, alpha Polypeptide III (P4HA3))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This P4HA3 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human P4HA3 (full length, N-term HIS tag) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product P4HA3 Protein
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Prolyl 4-Hydroxylase, alpha Polypeptide III (P4HA3) (AA 66-165) protein (GST tag)
P4HA3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Prolyl 4-Hydroxylase, alpha Polypeptide III (P4HA3) (AA 20-544) protein (His tag)Crystallography grade P4HA3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Prolyl 4-Hydroxylase, alpha Polypeptide III (P4HA3) (AA 25-542) protein (His tag)Crystallography grade P4HA3 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Prolyl 4-Hydroxylase, alpha Polypeptide III (P4HA3) (AA 20-544) protein (His-SUMO Tag)P4HA3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- P4HA3 (Prolyl 4-Hydroxylase, alpha Polypeptide III (P4HA3))
- Alternative Name
- p4ha3 (P4HA3 Products)
- Synonyms
- si:dkey-260j18.3 Protein, zgc:103728 Protein, D930031A02Rik Protein, prolyl 4-hydroxylase, alpha polypeptide III Protein, prolyl 4-hydroxylase subunit alpha 3 Protein, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III Protein, p4ha3 Protein, P4HA3 Protein, P4ha3 Protein
- Background
- This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants.
- Molecular Weight
- 59.3 kDa
- NCBI Accession
- NP_878907
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