PLEKHM2 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2729169

Product Details

Target: PLEKHM2 (Pleckstrin Homology Domain Containing, Family M (With RUN Domain) Member 2 (PLEKHM2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PLEKHM2 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human PLEKHM2 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PLEKHM2 (Pleckstrin Homology Domain Containing, Family M (With RUN Domain) Member 2 (PLEKHM2))

Alternative Name: Plekhm2 (PLEKHM2 Products)

Synonyms: zgc:194971 Protein, RP11-169K16.1 Protein, SKIP Protein, 2310034J19Rik Protein, AI854247 Protein, mKIAA0842 Protein, pleckstrin homology and RUN domain containing M2 Protein, pleckstrin homology domain containing, family M (with RUN domain) member 2 Protein, PLEKHM2 Protein, plekhm2 Protein, Plekhm2 Protein

Background: This gene encodes a protein that binds the plus-end directed microtubule motor protein kinesin, together with the lysosomal GTPase Arl8, and is required for lysosomes to distribute away from the microtubule-organizing center. The encoded protein belongs to the multisubunit BLOC-one-related complex that regulates lysosome positioning. It binds a Salmonella effector protein called Salmonella induced filament A and is a critical host determinant in Salmonella pathogenesis. It has a domain architecture consisting of an N-terminal RPIP8, UNC-14, and NESCA (RUN) domain that binds kinesin-1 as well as the lysosomal GTPase Arl8, and a C-terminal pleckstrin homology domain that binds the Salmonella induced filament A effector protein. Naturally occurring mutations in this gene lead to abnormal localization of lysosomes, impaired autophagy flux and are associated with recessive dilated cardiomyopathy and left ventricular noncompaction.

Molecular Weight: 112.6 kDa

NCBI Accession: NP_055979