POLR2J3 Protein (Myc-DYKDDDDK Tag)
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- Target See all POLR2J3 Proteins
- POLR2J3 (Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This POLR2J3 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Polymerase (RNA) II (DNA directed) polypeptide J3 (POLR2J3) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product POLR2J3 Protein
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Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3) protein (His tag)
POLR2J3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3) (AA 1-115) protein (Strep Tag)Crystallography grade POLR2J3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- POLR2J3 (Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))
- Abstract
- POLR2J3 Products
- Synonyms
- POLR2J2 Protein, RPB11b1 Protein, RPB11b2 Protein, RNA polymerase II subunit J3 Protein, POLR2J3 Protein
- Background
- This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo.
- Molecular Weight
- 12.9 kDa
- NCBI Accession
- NP_001091084
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