POT1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2729370

Product Details

Target: POT1 (Protection of Telomeres 1 (POT1))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This POT1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human POT1 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Su, Cheng, Tzeng, Lin, Hsu: "An H2A Histone Isotype, H2ac, Associates with Telomere and Maintains Telomere Integrity." in: PLoS ONE, Vol. 11, Issue 5, pp. e0156378, (2016) (PubMed).

Choi, Lakamp-Hawley, Kolar, Yan, Borgstahl, Ouellette: "The OB-fold domain 1 of human POT1 recognizes both telomeric and non-telomeric DNA motifs." in: Biochimie, Vol. 115, pp. 17-27, (2015) (PubMed).

Target Details

Target: POT1 (Protection of Telomeres 1 (POT1))

Alternative Name: Pot1 (POT1 Products)

Synonyms: HPOT1 Protein, POT1 Protein, DKFZp469P1825 Protein, pot1 Protein, 1500031H18Rik Protein, AI851169 Protein, Pot1 Protein, Pot1a Protein, protection of telomeres 1 Protein, protection of telomeres 1 L homeolog Protein, protection of telomeres 1A Protein, POT1 Protein, pot1.L Protein, Pot1a Protein, Pot1 Protein

Background: This gene is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described.

Molecular Weight: 71.3 kDa

NCBI Accession: NP_056265

Pathways: Cell Division Cycle, Telomere Maintenance